聂文锋，男，安徽安庆人，博士，教授，泰山学者青年专家，研究方向为植物表观遗传学与设施番茄生长发育调控。
2024-至今 山东农业大学，园艺科学与工程学院，教授
2020-2024扬州大学园艺系，校拔尖人才，校聘教授
2017-2020中国科学院上海逆境生物学研究中心，助理研究员
2015-2017中国科学院上海生命科学研究院，博士后
2012-2014美国普渡大学，园艺与景观设计系，联合培养博士研究生
2009-2014浙江大学，农业与生物技术学院，蔬菜学博士
2005-2009吉林大学，植物科学学院，园艺学士
主要研究内容：
DNA序列上的甲基化修饰（5mC）和组蛋白H2A.Z变体是两种重要且保守的表观遗传修饰，二者在植物全基因组整体层面相互拮抗而在局部特异性DNA序列上相互协同，进而对植物细胞的基因表达调控产生重要影响。5mC和H2A.Z等表观遗传修饰可充分释放植物表型可塑性的潜力，在调控番茄等蔬菜作物的生长发育、抗逆性和品质形成等过程中发挥重要作用。
DNA主动去甲基化是维持细胞5mC含量和分布的重要生物学过程，决定基因是否表达和表达水平。本人主要围绕番茄的经济性状（产量、品质和活性成分）形成过程中的环境塑造机制及其表观遗传调控，深入研究DNA去甲基化和组蛋白结构变化（如H2A.Z变体、乙酰化修饰等）在番茄植株对盐碱、低温、弱光等逆境胁迫的应答中以及在果实发育和功能活性物质积累中的机制、功能和应用，为优化设施蔬菜栽培提供新理论和新方法。
所在设施蔬菜抗性与品质调控团队每年招收蔬菜生理与分子生物学方向的硕、博研究生，博士后，欢迎老师、同学来实验室交流！
主要科研项目：
（1）国家自然科学基金委员会，面上项目，弱光环境下组蛋白H2A.Z变体调控番茄株型的表观遗传机制，2025/01-2028/12，主持；
（2）山东省级人才工程，泰山学者青年专家项目，基于DNA去甲基化的番茄表观遗传种质构建及应用研究，2025/01-2027/12，主持；
（3）山东农业大学，人才引进科研启动经费（“冲一流”建设专项经费），2024/09-2029/08主持；
（4）国家自然科学基金委员会，青年科学基金项目，32002046，DNA去甲基化酶CsDML4调控水苏糖合成酶基因CsSTS表达和黄瓜果实膨大的机理，2021/01-2023/12主持；
（5）江苏省科学技术厅，江苏省自然科学青年基金项目，组蛋白变体H2A.Z调控番茄植株对亚低温响应的机理，2020/07-2023/06，主持;
（6）国家自然科学基金委员会，面上项目，番茄根结线虫抗性反应中redox介导的温敏性及其调控机制，2013/01-2016/12，参与。
主要学术论文（#为共同第一作者，*为通讯作者）：
(1) Nie, W.F.*, Mao, Y.Y., Xin E. and Liu, R.* Actin-related protein ARP4 and ARP6 antagonistically regulate DNA demethylase ROS1 in plants. Plant Physiology, 2024, 195(1):279-282.
(2) Nie, W.F#, Li, Y.#, Chen, Y., Guang, Y., Zhou, Y.,* and Yang, Y.*Spectrum-dependent environmental regulation guides the morphogenesis, architecture, and flowering of pepper plants. Journal of Photochemistry and Photobiology B-Biology, 2023, 241:112673.
(3) Zhou, X.#, Wei, M.#, Nie, W.F.#, Xi, Y., Li, P., Zheng, Tang, Q.,K., Satheesh, V., Wang, Y., Luo, J., Du, X., Liu, R., Yang, Z., La, H., Zhong, Y., Yang, Y., Zhu, J.K., Du, J.* and Lei, M.* The H3K9me2-binding protein AGDP3 limits DNA methylation and transcriptional gene silencing in Arabidopsis. Journal of Integrative Plant Biology, 2022, 64(12):2385-2395.
(4) Xu, A., Wei, N., Zhou, S., Hu, H., Bie, Z., Nie, W.F.*, Cheng, F.*. Thioredoxin h2 inhibits MAPK3 phosphorylation to regulate the CBF2 COR signaling pathway in response to chilling stress in Citrullus lanatus. Horticulture Research, 2022, 10(2):uhac256.
(5) Yang, X, Zhang, X, Yang, Y, Zhang, H, Zhu, W.* and Nie, W.F.*Histone variant Sl_H2A.Z regulates the carotenoids biosynthesis and gene expression during the tomato fruit ripening. Horticulture Research, 2021, 8, 58.
(6) Liu, P. #, Nie W.F. # *, Xiong, X., Wang, Y., Jiang, Y., Huang, P., Lin, X., Qin, G., Huang, H., Niu, Q., Du, J., Lang, Z., Lozano-Duran R. and Zhu, J.K.(*)A novel protein complex that regulates active DNA demethylation in Arabidopsis. Journal of Integrative Plant Biology,2021, doi:10.1111/jipb.13045.
(7) Jiang, Y.#, Ding, X.#, Wang, J., Zou, J. and Nie, W.F.*Decreased low-light regulates plant morphogenesis through the manipulation of hormone biosynthesis in Solanum lycopersicum. Environmental and Experimental Botany,2021, 185.
(8) Nie, W.F.*DNA methylation: from model plants to vegetable crops. Biochemical Society Transactions, 2021, BST20210353. doi: https://doi.org/10.1042/BST20210353
(9) Wang, J.#, Feng, Y.#, Ding, X.#, Huo, J., Nie, W.F.* Identification of long non-coding RNAs associated with tomato fruit expansion and ripening by strand-specific paired-end RNA Sequencing. Horticulturae, 2021, 7, 522.
(10) Yang, X.#, Liu, Y.#, Zhang, H.#, Wang, J., Zinta, G., Xie, S., Zhu W.* and Nie, W.F.* Genome-wide identification of circular RNAs in response to low-temperature stress in tomato leaves. Frontiers in Genetics, 2020, 11.
(11) Nie, W.F.#, Lei, M. #, Zhang, M., Tang, K., Huang, H., Zhang, C., Miki, D., Liu, P., Yang, Y., Wang, X., Zhang, H., Lang, Z., Liu, N., Xu, X., Yelagandula, R., Zhang, H., Wang, Z., Chai, X., Andreucci, A., Yu, J.Q., Berger, F., Lozano-Duran, R. and Zhu, J.K.*. Histone acetylation recruits the SWR1 complex to regulate active DNA demethylation in Arabidopsis. Proc Natl Acad Sci USA, 2019, 116 (33): 16641-16650.
(12) Nie W.F.*, Wang M.M., Xia X.J., Zhou Y.H., Shi K., Chen Z.X. and Yu J.Q. (*). Silencing of tomato RBOH1 and MPK2 abolishes brassinosteroid-induced H2O2 generation and stress tolerance, Plant Cell and Environment, 2013, 36: 789-803.
(13) 聂文锋*; 王金玉; 高春娟; 陈学好*; 表观遗传修饰调控园艺植物果实发育的研究进展, 园艺学报, 2022, 49 (3): 671-686.
(14) Lei, M.#, La, H.G.#, Lu, K., Wang, P., Miki, D., Ren, Z., Duan, C.G., Wang, X., Tang, K., Zeng, L., Yang, L., Zhang, H., Nie, W.F., Liu, P., Zhou, J., Liu, R., Zhong, Y., Liu, D. and Zhu, J.K.* Arabidopsis EDM2 promotes IBM1 distal polyadenylation and regulates genome DNA methylation patterns. Proc Natl Acad Sci USA, 2014, 111, 527-532
(15) Lang, Z.#, Lei, M.#, Wang, X., Tang, K., Miki, D., Zhang, H., Mangrauthia, S., Liu, W., Nie, W.F., Ma, G., Yan, J., Duan, C.G., Hsu, C.C., Wang, C., Tao, W., Gong, Z. and Zhu, J.K.* The methyl-CpG-binding protein MBD7 prevents DNA hypermethylation and transcriptional gene silencing by facilitating active DNA demethylation. Molecular Cell, 2015, 57, 971-983.
(16) Yang, Y., La, H., Tang, K., Miki, D., Yang, L., Wang, B., Duan, C.G., Nie, W.F., Wang, X., Wang, S., Pan, Y., Tran, E.J., An, L., Zhang, H.* and Zhu, J.K.* SAC3B, a central component of the mRNA export complex TREX-2, is required for prevention of epigenetic gene silencing in Arabidopsis. Nucleic Acids Research, 2017, 45, 181-197.
(17) Yang, Y.#, Tang, K.#, Datsenka, T.U.#, Liu, W., Lv, S., Lang, Z., Wang, X., Gao, J., Wang, W., Nie, W.F., Chu, Z., Zhang, H., Handa, A. K., Zhu, J. K. and Zhang, H. Critical function of DNA methyltransferase 1 in tomato development and regulation of the DNA methylome and transcriptome. Journal of Integrative Plant Biology, 2019, 61(12): 1224-1242
(18) Zhao, C.Z.#*, Jiang, W.#, Zayed, O.#, Liu, X., Tang, K., Nie, W.F., Li, Y.L., Xie, S.J., Li, Y., Long, T.D., Liu, L. L., Zhu, Y.F., Zhao, Y. and Zhu, J.K.* The LRXs-RALFs-FER module controls plant growth and salt stress responses by modulating multiple plant hormones. National Science Review, 2021, 8
(19) Huang, P.#, Huang, H.#, Lin, X.Q., Liu, P., Zhao, L., Nie, W.F., Zhu, J.K., and Lang, Z.B. MSI4/FVE is required for accumulation of 24-nt siRNAs and DNA methylation at a subset of target regions of RNA-directed DNA methylation. Plant Journal, 2021, 108, 347-357.
(20) Wang, Z.#, Wang M.#, Yang, C., Zhao, L., Qin, G., Peng, L., Zheng, Q., Nie, W.F., Song, C.P., Shi, H., Zhu, J.K.* and Zhao, C.*. SWO1 modulates cell wall integrity under salt stress by interacting with importin ɑ in Arabidopsis. Stress Biology, 2021, 1:9
联系方式：
nwf2024@sdau.edu.cn，国重楼729
