Scientific Research
We are interested in novel microbial enzymes invovled in nucleic acids metabolism and the development of enzymatic tools for molecular biotechnology and medicine.
Directions include but not limited to:
Novel single-subunit RNA polymerases--In vitro RNA synthesis;
Novel DNA polymerases--DNA amplification, sequencing and storage;
Novel reverse transcriptase--RNA sequencing and IVD;
Novel nucleases--DNA/RNA processing, modification and genome editing;
Unknown nucleic acids enzymes from bacteriophages and viruses--The dark matter to modern molecular biology, NOTHING IS IMPOSSIBLE!
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[1] Yu B,Chen Y.DNA-terminus-dependent transcription by T7 RNA polymerase and its C-helix mutants.Nucleic Acids Research.2024
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[2] Yan Y,Xiao J,Xian W.Phage defense system CBASS is regulated by a prokaryotic E2 enzyme that imitates the ubiquitin pathway..Nature Microbiology.2024
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[3] Wang X.SARS-CoV-2 nsp15 preferentially degrades AU-rich dsRNA via its dsRNA nickase activity..Nucleic Acids Research.2024
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[4] Li J,Cheng R,Wang Z.Structures and activation mechanism of Gabija anti-phage system.Nature.2024(Accelerated Article Preview publication)
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[5] Lu X.The nuclease-associated short prokaryotic Argonaute system nonspecifically degrades DNA upon activation by target recognition..Nucleic Acids Res..2023:1-12
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