Recently,
Ph.D. student Ruihua Tang from BEBC has published his first author research
paper in Lab on a chip entitled " A Fully Disposable and
Integrated Paper-based Device
for Nucleic Acid Extraction,
Amplification and Detection".
To overcome
the challenge of paper-based sample-to answer detection, this paper developed a
fully disposable and integrated paper-based sample-in-answer-out device for NAT by integrating nucleic acid extraction, helicase-dependent isothermal
amplification and lateral flow assay detection into one paper device.
This simple device allows on-chip dried reagent storage and equipment-free
nucleic acid amplification with simple operation steps, which could be
performed by untrained users in remote settings. The proposed device consists
of sponge-based reservoir and
paper-based valve for nucleic acid extraction, integrated battery, PTC
ultrathin heater, temperature control switch and on-chip dried enzyme mix
storage for isothermal amplification, and lateral flow test strip for naked-eye
detection. It can sensitively detect Salmonella typhimurium, as a model target, with a detection limit of
as low as 102 CFU/ml in wastewater and egg, and 103 CFU/ml in milk and juice in about an hour. This fully disposable and
integrated paper-based device holds great potential for future POC applications
in resource-limited settings.
The work is
under the guidance of Feng Xu Professor. The co-authors of the paper include
Yan Gong, MinLi You, Zhi Liu, Jane
Ru Choi, Ting Wen, Zhiguo Qu, Hui Yang,
Qibing Mei, Feng Xu.
Links: http://pubs.rsc.org/-/content/articlehtml/2017/lc/c6lc01586g
