Jun Yan, Xi Chen, Fengshan Wang* and Hongzhi Cao*

Org. Biomol. Chem., 2013, 11, 842-848

ABSTRACT

Fluorinated Thomsen–Friedenreich (T) antigens were synthesized efficiently from chemically produced fluorinated monosaccharides using a highly efficient one-pot two-enzyme chemoenzymatic approach containing a galactokinase and a D-galactosyl-β1–3-N-acetyl-D-hexosamine phosphorylase. These fluorinated T-antigens were further sialylated to form fluorinated ST-antigens using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α-2–3-sialyltransferase