Indexed by: Journal paper

First Author: Yang Li，Zhaowei Xu，Qing Lei，Dan-yun Lai，Hongyan Hou

Correspondence Author: Feng Wang,Xionglin Fan,Sheng-ce Tao

Co-author: He-wei Jiang,Yun-xiao Zheng,Xue-ning Wang,Jiaoxiang Wu,Ming-liang Ma,Bo Zhang,Hong Chen,Caizheng Yu,Jun-biao Xue,Hai-nan Zhang,Huan Qi,Shu-juan Guo ,Yandi Zhang,Xiaosong Lin,Zongjie Yao,Huiming Sheng,Ziyong Sun

Journal: Cell Reports

Affiliation of Author(s): 华中科技大学

Discipline: Medicine

First-Level Discipline: Basic Medicine

Document Type: J

Volume: 36

Issue: 2

ISSN No.: 2211-1247

Key Words: COVID-19; SARS-CoV-2; humoral immunity; immune response; non-structural/accessory proteins; proteome microarray

DOI number: 10.1016/j.celrep.2021.109391

Date of Publication: 2021-06-26

Impact Factor: 9.423

Teaching and Research Group: 华中科技大学同济医学院基础医学院病原生物学

Abstract: The immunogenicity of the SARS-CoV-2 proteome is largely unknown, especially for non-structural proteins and accessory proteins. In this study, we collect 2,360 COVID-19 sera and 601 control sera. We analyze these sera on a protein microarray with 20 proteins of SARS-CoV-2, building an antibody response landscape for immunoglobulin (Ig)G and IgM. Non-structural proteins and accessory proteins NSP1, NSP7, NSP8, RdRp, ORF3b, and ORF9b elicit prevalent IgG responses. The IgG patterns and dynamics of non-structural/accessory proteins are different from those of the S and N proteins. The IgG responses against these six proteins are associated with disease severity and clinical outcome, and they decline sharply about 20 days after symptom onset. In non-survivors, a sharp decrease of IgG antibodies against S1 and N proteins before death is observed. The global antibody responses to non-structural/accessory proteins revealed here may facilitate a deeper understanding of SARS-CoV-2 immunology.