Indexed by: Journal paper

First Author: Yang Li，Dan-yun Lai，Qing Lei，Zhao-wei Xu，Feng Wang，Hongyan Hou

Correspondence Author: Hong Shan，Xionglin Fan，Sheng-ce Tao

Co-author: Lingyun Chen, Jiaoxiang Wu, Yan Ren, Ming-liang Ma, Bo Zhang, Hong Chen, Caizheng Yu, Jun-biao Xue, Yun-xiao Zheng, Xue-ning Wang, He-wei Jiang, Hai-nan Zhang, Huan Qi, Shu-juan Guo, Yandi Zhang, Xiaosong Lin, Zongjie Yao, Pengfei Pang, Dawei Shi, Wei Wang, Xiao Yang, Jie Zhou, Huiming Sheng,Ziyong Sun

Journal: Cellular & molecular immunology

Affiliation of Author(s): 华中科技大学

Discipline: Medicine

First-Level Discipline: Basic Medicine

Document Type: J

Volume: 18

Issue: 3

Page Number: 621-631

ISSN No.: 2042-0226

Key Words: Antibody; Diagnosis; SARS-CoV-2; Spike; peptide.

DOI number: 10.1038/s41423-020-00612-5

Date of Publication: 2021-01-22

Impact Factor: 11.53

Teaching and Research Group: 华中科技大学基础医学院病原生物学系医学微生物学

Abstract: Serological tests play an essential role in monitoring and combating the COVID-19 pandemic. Recombinant spike protein (S protein), especially the S1 protein, is one of the major reagents used for serological tests. However, the high cost of S protein production and possible cross-reactivity with other human coronaviruses pose unavoidable challenges. By taking advantage of a peptide microarray with full spike protein coverage, we analyzed 2,434 sera from 858 COVID-19 patients, 63 asymptomatic patients and 610 controls collected from multiple clinical centers. Based on the results, we identified several S protein-derived 12-mer peptides that have high diagnostic performance. In particular, for monitoring the IgG response, one peptide (aa 1148-1159 or S2-78) exhibited a sensitivity (95.5%, 95% CI 93.7-96.9%) and specificity (96.7%, 95% CI 94.8-98.0%) comparable to those of the S1 protein for the detection of both symptomatic and asymptomatic COVID-19 cases. Furthermore, the diagnostic performance of the S2-78 (aa 1148-1159) IgG was successfully validated by ELISA in an independent sample cohort. A panel of four peptides, S1-93 (aa 553-564), S1-97 (aa 577-588), S1-101 (aa 601-612) and S1-105 (aa 625-636), that likely will avoid potential cross-reactivity with sera from patients infected by other coronaviruses was constructed. The peptides identified in this study may be applied independently or in combination with the S1 protein for accurate, affordable, and accessible COVID-19 diagnosis.

Note: Y.Li, D. Lai, Q. Lei, etal. Systematic evaluation of SARS-CoV-2 spike protein derived peptides for diagnosis of COVID-19 patients. Cellular and Molecular Immunology, 2021,18:621-631.

Links to published journals: https://www.nature.com/articles/s41423-020-00612-5